adherent preadipocytes Search Results


adherent preadipocytes  (PromoCell)
93
PromoCell adherent preadipocytes
Adherent Preadipocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adherent preadipocytes/product/PromoCell
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adherent preadipocytes - by Bioz Stars, 2026-02
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enriched preadipocytes  (Miltenyi Biotec)
99
Miltenyi Biotec enriched preadipocytes
miRNA expression profiling during 3T3-L1 adipogenesis. A : Intensity scatter plot showing comparison of miRNA profiles between undifferentiated 3T3-L1 <t>preadipocytes</t> (day 0) and differentiated 3T3-L1 adipocytes (day 9). The 12 miRNAs that are the focus of our detailed analyses are highlighted in red and indicated by a label. B : Validation of miRNA array results for 12 regulated miRNAs by RT-PCR assays. Expressions for all miRNAs are plotted as fold-change in log2 scale; positive indicates enriched in day 9 vs. day 0. □, Array result ( n = 2); ■, RT-PCR result ( n = 4). Data are expressed as means ± SE. C : Comparison by RT-PCR of miRNA regulation during 3T3-L1 differentiation and primary fat cell development. Expressions of all miRNAs are normalized to internal control and plotted as fold-change in log2 scale. ■, day 9 vs. day 0, same data as B ( n = 4); □, mature adipocytes vs. enriched preadipocytes ( n = 4). Data are expressed as mean ± SE. D : Intronic miRNAs and their host genes are co-regulated during adipogenesis. miR-422b is in the intron of PPARGC1b ; miR-107 is in the intron of PANK1 ; the two copies of miR-103 are in the introns of PANK2 and PANK3 . miRNA expression levels are measured by RT-PCR and normalized to internal control. Expression levels of mRNAs are also determined by RT-PCR and normalized to internal control. Expressions are shown as fold-change relative to the level at day 0. □, day 0; ■, day 2; ▨, day 4. Data are expressed as mean ± SE ( n = 3).
Enriched Preadipocytes, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enriched preadipocytes/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
enriched preadipocytes - by Bioz Stars, 2026-02
99/100 stars
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miRNA expression profiling during 3T3-L1 adipogenesis. A : Intensity scatter plot showing comparison of miRNA profiles between undifferentiated 3T3-L1 preadipocytes (day 0) and differentiated 3T3-L1 adipocytes (day 9). The 12 miRNAs that are the focus of our detailed analyses are highlighted in red and indicated by a label. B : Validation of miRNA array results for 12 regulated miRNAs by RT-PCR assays. Expressions for all miRNAs are plotted as fold-change in log2 scale; positive indicates enriched in day 9 vs. day 0. □, Array result ( n = 2); ■, RT-PCR result ( n = 4). Data are expressed as means ± SE. C : Comparison by RT-PCR of miRNA regulation during 3T3-L1 differentiation and primary fat cell development. Expressions of all miRNAs are normalized to internal control and plotted as fold-change in log2 scale. ■, day 9 vs. day 0, same data as B ( n = 4); □, mature adipocytes vs. enriched preadipocytes ( n = 4). Data are expressed as mean ± SE. D : Intronic miRNAs and their host genes are co-regulated during adipogenesis. miR-422b is in the intron of PPARGC1b ; miR-107 is in the intron of PANK1 ; the two copies of miR-103 are in the introns of PANK2 and PANK3 . miRNA expression levels are measured by RT-PCR and normalized to internal control. Expression levels of mRNAs are also determined by RT-PCR and normalized to internal control. Expressions are shown as fold-change relative to the level at day 0. □, day 0; ■, day 2; ▨, day 4. Data are expressed as mean ± SE ( n = 3).

Journal: Diabetes

Article Title: MicroRNAs Induced During Adipogenesis that Accelerate Fat Cell Development Are Downregulated in Obesity

doi: 10.2337/db08-1299

Figure Lengend Snippet: miRNA expression profiling during 3T3-L1 adipogenesis. A : Intensity scatter plot showing comparison of miRNA profiles between undifferentiated 3T3-L1 preadipocytes (day 0) and differentiated 3T3-L1 adipocytes (day 9). The 12 miRNAs that are the focus of our detailed analyses are highlighted in red and indicated by a label. B : Validation of miRNA array results for 12 regulated miRNAs by RT-PCR assays. Expressions for all miRNAs are plotted as fold-change in log2 scale; positive indicates enriched in day 9 vs. day 0. □, Array result ( n = 2); ■, RT-PCR result ( n = 4). Data are expressed as means ± SE. C : Comparison by RT-PCR of miRNA regulation during 3T3-L1 differentiation and primary fat cell development. Expressions of all miRNAs are normalized to internal control and plotted as fold-change in log2 scale. ■, day 9 vs. day 0, same data as B ( n = 4); □, mature adipocytes vs. enriched preadipocytes ( n = 4). Data are expressed as mean ± SE. D : Intronic miRNAs and their host genes are co-regulated during adipogenesis. miR-422b is in the intron of PPARGC1b ; miR-107 is in the intron of PANK1 ; the two copies of miR-103 are in the introns of PANK2 and PANK3 . miRNA expression levels are measured by RT-PCR and normalized to internal control. Expression levels of mRNAs are also determined by RT-PCR and normalized to internal control. Expressions are shown as fold-change relative to the level at day 0. □, day 0; ■, day 2; ▨, day 4. Data are expressed as mean ± SE ( n = 3).

Article Snippet: Primary adipocytes were obtained after collagenase/dispase digestion, and enriched preadipocytes (CD11b − CD90 − CD31 − ) were isolated by a negative selection on an autoMACS Separator according to the manufacturer's instruction (Miltenyi Biotec) (Supplementary Fig. S1, available in an online appendix at http://diabetes.diabetesjournals/cgi/content/full/db08-1299/DC1 ).

Techniques: Expressing, Comparison, Biomarker Discovery, Reverse Transcription Polymerase Chain Reaction, Control

Inverse correlation of miRNA expression during adipogenesis and obesity. A : Scatter plot showing the inverse correlation of miRNA regulation during adipogenesis and obesity. A total of 79 miRNAs, whose levels are above the mean expression of all miRNAs in at least one of the four samples (undifferentiated 3T3-L1 preadipocytes [day 0], differentiated 3T3-L1 adipocytes [day 9], and adipocytes from wild-type [WT] and ob/ob mice) are shown. Fold-change is based on array result and plotted in a log2 scale. The 12 miRNAs that are the focus of our detailed analyses are highlighted in red and indicated by a label. Pearson's correlation coefficient: r = −0.51, P < 0.0001. B : Fisher's exact test for 79 miRNAs is shown in A . A total of 41 miRNAs were upregulated during differentiation (Diff_Up), among which 31 miRNAs were downregulated during obesity (Ob_Down), whereas 10 miRNAs were upregulated (Ob_Up). A total of 38 miRNAs were downregulated during differentiation (Diff_Down), among which 26 miRNAs were upregulated during obesity (Ob_Up), whereas 12 miRNAs were downregulated (Ob_Down). P = 0.0001 by Fisher's exact test.

Journal: Diabetes

Article Title: MicroRNAs Induced During Adipogenesis that Accelerate Fat Cell Development Are Downregulated in Obesity

doi: 10.2337/db08-1299

Figure Lengend Snippet: Inverse correlation of miRNA expression during adipogenesis and obesity. A : Scatter plot showing the inverse correlation of miRNA regulation during adipogenesis and obesity. A total of 79 miRNAs, whose levels are above the mean expression of all miRNAs in at least one of the four samples (undifferentiated 3T3-L1 preadipocytes [day 0], differentiated 3T3-L1 adipocytes [day 9], and adipocytes from wild-type [WT] and ob/ob mice) are shown. Fold-change is based on array result and plotted in a log2 scale. The 12 miRNAs that are the focus of our detailed analyses are highlighted in red and indicated by a label. Pearson's correlation coefficient: r = −0.51, P < 0.0001. B : Fisher's exact test for 79 miRNAs is shown in A . A total of 41 miRNAs were upregulated during differentiation (Diff_Up), among which 31 miRNAs were downregulated during obesity (Ob_Down), whereas 10 miRNAs were upregulated (Ob_Up). A total of 38 miRNAs were downregulated during differentiation (Diff_Down), among which 26 miRNAs were upregulated during obesity (Ob_Up), whereas 12 miRNAs were downregulated (Ob_Down). P = 0.0001 by Fisher's exact test.

Article Snippet: Primary adipocytes were obtained after collagenase/dispase digestion, and enriched preadipocytes (CD11b − CD90 − CD31 − ) were isolated by a negative selection on an autoMACS Separator according to the manufacturer's instruction (Miltenyi Biotec) (Supplementary Fig. S1, available in an online appendix at http://diabetes.diabetesjournals/cgi/content/full/db08-1299/DC1 ).

Techniques: Expressing